More than one-third of the plant species of Waterloo Region are found within Cruickston Charitable Research Reserve with approximately 600 species. Of these, 48 species are on the significant species list of native vascular plants for the Regional Municipality of Waterloo. In addition, in 2002, two species not previously known in Waterloo Region and presumed to be candidates for the list of Regionally Significant plants, were found.

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